UTILIZING SPERMATOZOA WITH THE HIGHEST GENOMIC INTEGRITY ENHANCES ICSI OUTCOME

To demonstrate that selecting spermatozoa with the highest genomic integrity enhances ICSI outcomes. Over last 4 years, consenting men undergoing ART provided ejaculates for sperm chromatin fragmentation (SCF) screening before and after density gradient centrifugation (DGC). In a subsequent cycle, this assay was carried out microfluidics selection (MFSS). SCF assessed by terminal deoxynucleotidyl transferase dUTP nick-end labeling (TUNEL); at least 500 were per sample normal threshold of ≤15%. Ejaculated specimens analyzed in standard fashion used DGC or MFSS. Fertilization clinical outcome recorded compared between sperm-processing methods. A total 21 (43.3±8 years) had an average their raw semen 22.1±10%, which decreased to 19.1±7% DGC. These underwent 39 cycles female partners (38.0±4 years), achieving fertilization rate 57.0% (167/293), implantation only 5.1% (4/78), pregnancy (CPR) 11.8% (4/34), half resulting loss (2/4). Subsequently, these couples 26 The 1.2±1%, remarkably lower than specimen (P<0.05). cycles, there improved 69.3% (167/241; P<0.05). rose 30.8% (16/52; P<0.0001) CPR increased 53.8% (14/26; P<0.001); all are ongoing have delivered (P<0.0001). Based on results, 55 (42.3±8 elevated ejaculate solely selected MFSS treated 69 (38.3±5 years). samples 22.3±10%, fell 3.0±4% following led 75.9% (456/605) rate, 24.8% (36/145), 47.8% (32/67), 41.8% (28/67) pregnancies. Compared more conventional DGC, is capable most progressively motile integrity. Treatment high DNA fertilization, embryo development,